Composite

Part:BBa_K2224002:Design

Designed by: Yunchang Zhang   Group: iGEM17_SMS_Shenzhen   (2017-10-24)


Promoter+RBS+Lecanicillium lecanii strain CA-12 cuticle-degrading proteinase (Pr1)+Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 101
    Illegal AgeI site found at 462
    Illegal AgeI site found at 1128
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

1. Since Lecanicillium lecanii is fungus, the possibility of this proteinase to be expressed and modified successfully in E.coli was unknown. Then, we looked for past scientific papers and found out that there were examples of E.coli expressing this enzyme.*1; 2. Since this enzyme is from the Lecanicillium lecanii, a organism that feeds on scale insect, we supposed that this proteinase might show a strong ability in degradation. We then conducted experiments under the SEM to get a qualitative measurement.


Source

genomic sequence of Lecanicillium lecanii strain CA-12

References